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Fig. 7 | Cancer Cell International

Fig. 7

From: TP53 mutant cell lines selected for resistance to MDM2 inhibitors retain growth inhibition by MAPK pathway inhibitors but a reduced apoptotic response

Fig. 7

The effect of trametinib treatment on paired SJSA-1 and SN40R2. a Growth inhibition curves by SRB assay of SJSA-1 and SN40R2 treated with trametinib for 72 h. b Immunobloting of SJSA-1 and SN40R2 after trametinib for 6 h. Cell cycle distribution changes for 24 (c) and 48 (d) hour treatment with trametinib measured by FACS analysis. Sub-G1 events by FACS (e) and caspase 3/7 activity (f) after 24 h treatment, as indicators of apoptosis. Statistically significant p-values (*p < 0.05) are indicated where appropriate. Data are presented as mean ± standard error of mean (SEM) for at least three independent repeats

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Cancer Cell International

ISSN: 1475-2867

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