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Fig. 3 | Cancer Cell International

Fig. 3

From: Tumor-associated macrophages modulate resistance to oxaliplatin via inducing autophagy in hepatocellular carcinoma

Fig. 3

Co-culturing with macrophages activate autophagy in HCC cells. a, b SMMC-7721 and Huh-7 cells stably expressing the GFP-LC3 fusion protein were established and then respectively co-cultured with macrophages. GFP-LC3–positive dots per transfected cell were determined in 3 independent experiments. Eight random fields (×200) representing 200 cells were counted. Data shown are mean (SD) from at least 3 independent experiments. Means were compared between two groups using unpaired, two-tailed Student’s t-test. * P < 0.05, ** P < 0.01. c, d Western blot analysis of LC3-I and LC3-II in SMMC-7721 and Huh-7 cells after co-culturing with macrophages. Scale bars, 100 μm

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