Fig. 4

In vitro Hep-2 and Tu212 cells exposed to pepsin after the blockage of IL8 receptors. a Western blot analysis of E-cadherin, vimentin, β-catenin, snail, and slug expression in Hep-2 and Tu212 cells exposed to pepsin with or without SB225002 treatment to inhibit the IL-8 receptor CXCR2. b Effect of pepsin on Hep-2 and Tu212 cell proliferation with or without SB225002 treatment to inhibit the IL-8 receptor CXCR2. Cell proliferation was measured using Edu assays (magnification, ×100). c Representative histograms of cell cycle assays showing the percentage of pepsin-treated Hep-2 and Tu212 cells in S phase with or without SB225002 treatment to inhibit the IL-8 receptor CXCR2. d Migratory properties of Hep-2 and Tu212 cells exposed to pepsin with or without inhibition of the IL-8 receptor CXCR2 by SB225002 treatment analyzed using transwell migration assays, with the number of migrated Hep-2 and Tu212 cells shown (magnification, ×200). *P < 0.05 compared to that of the controls