Fig. 3

Knockdown of USP24 but not USP9X induces apoptosis and growth inhibition of T-ALL cells. a, b Jurkat cells were transfected with control or specific USP9X siRNA lentiviral for 72 h followed by flow cytometry using Annexin V and western blot assays for indicated antibodies. c, d Jurkat cells were transfected with control or specific USP24 siRNA lentiviral for 72 h followed by flow cytometry using Annexin V and western blot assays for indicated antibodies. e The mRNA level of USP9X and USP24 was assessed by RT-PCR. f USP9X was over-expressed in 293T and treated in the presence or absence of MG132, then the indicated protein were determined by western blotting. g Cell viability was monitored by trypan blue staining. All the date shown is representative of values from at least three independent experiments with similar result. **P < 0.01 vs. control group