Fig. 5
From: TRIM52 promotes colorectal cancer cell proliferation through the STAT3 signaling
TRIM52 modulates the STAT3 signaling pathway. a The levels of p-STAT3 and STAT3 in SW480 and LoVo cells transduced with indicated virus for 48 h were analyzed by immunoblot assay. b The levels of PTP1B, TCPTP, SHP1 and SHP2 in SW480 and LoVo cells transduced with indicated virus for 48 h. c SW480 cell lysates were immunoprecipitated (IP) with anti-SHP2 (Ab) or immunoglobulin G (IgG), and immunoblotted (IB) with anti-TRIM52 or anti-SHP2. Input: total cell lysate. d SW480 cells were transduced with TRIM52 expression lentivirus (TRIM52OE) or control Vector lentivirus. Cell lysates were IP with anti-SHP2 (Ab) or IgG, and IB with anti-Ubiquitin (Ub). e The levels of TRIM52, SHP2, p-STAT3 and STAT3 in xenograft tumors were analyzed by immunoblot assay