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Fig. 1 | Cancer Cell International

Fig. 1

From: MKP-4 suppresses hepatocarcinogenesis by targeting ERK1/2 pathway

Fig. 1

MKP-4 interacts with ERK1/2 in liver tumor cells and tissues. a The results of mass spectrometry in HCC tissues. b Verification of the interaction between MKP-4 and ERK1/2 in HCC tissues using immunoprecipitation assay. c Reciprocal immunoprecipitation of MKP-4 and ERK1/2 in HepG2 cells. Lysates of HepG2 cells were immunoprecipitated with anti-MKP-4, anti-ERK1/2 antibodies or control IgG. The immunoprecipitates were subjected to western blot analysis with anti-ERK1/2 and anti-MKP-4 antibodies. d Immunofluorescence analysis of MKP-4 and ERK1/2 in HepG2 cells. HepG2 cells were subjected to immunofluorescence assay using anti-MKP-4 and anti-ERK1/2 antibodies. Scale bar: 50 μm

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