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Fig. 2 | Cancer Cell International

Fig. 2

From: Melatonin enhances TNF-α-mediated cervical cancer HeLa cells death via suppressing CaMKII/Parkin/mitophagy axis

Fig. 2

Melatonin amplified caspase-9-dependent mitochondrial apoptosis. a, b The change of mitochondrial ROS (mROS) was determined via flow cytometry. Melatonin augmented the cellular oxidative stress in the presence of TNF-α. c mPTP opening was determined to with the help of ELISA under TNF-α and/or melatonin treatment. d, e JC-1 probe was used to observe the changes in mitochondrial membrane potential. The red fluorescence indicated the normal mitochondria with high membrane potential. The green fluorescence was the marker of damaged mitochondria with reduced membrane potential. f, g Cyt-c translocation from mitochondria into nucleus was monitored via immunofluorescence. The expression of nuclear cyt-c was determined. h, i NAO probe was used to observe the content of non-oxidized cardiolipin. *P < 0.05 vs. ctrl group, #P < 0.05 vs. TNF-α group

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