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Fig. 4 | Cancer Cell International

Fig. 4

From: Melatonin enhances TNF-α-mediated cervical cancer HeLa cells death via suppressing CaMKII/Parkin/mitophagy axis

Fig. 4

Melatonin regulated mitophagy to enhance cellular apoptosis in response to TNF-α treatment. a The overlap of mitochondria and lysosome in the HeLa cells under TNF-α treatment in the presence of melatonin or not. Mitophagy was activated by TNF-α treatment and was suppressed by melatonin supplementation. b The number of mitophagy in each cell was recorded. cf Western blotting was used to analyzd the expression of mitophagy-related parameters. g, h ELISA assay was used to observe the activity of caspase-3 and caspase-9 activity. FCCP (5 μM) was used to activate the mitophagy 2 h before the melatonin and TNF-α treatment. *P < 0.05 vs. ctrl group, #P < 0.05 vs. TNF-α group, @P < 0.05 vs. TNF-α + melatonin group

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