Fig. 6

Silence of ST6GAL NAC1 inhibits Akt tumorigenicity of OCSCs in vivo. a In vivo limiting dilution assay was used to detect in vivo stem cell ratio of ST6GALNAC1 in mice; b the tumor formed by OCSCs in nude mice were examined. c Quantification of tumor volumes in b; d Quantification of tumor weights in b; e β-catenin staining intensity in OCSCs detected by immunohistochemical staining (×200). The measurement data were expressed as mean ± standard deviation. The in vivo stem cell ratio of ST6GALNAC1 in mice and quantification of tumor volumes were analyzed by t-test. The quantification of tumor weights was examined by Two-way ANOVA (n = 6). *p < 0.05 vs. the si-NC group; ^#p < 0.05 vs. the empty vector group; the experiment was repeated 3 times