Fig. 8

Inactivation of the Akt signaling pathway suppresses proliferation, migration and invasion and characteristics acquisition of OCSCs by ST6GALNAC1. a Detection of CD44, Nanog, Oct4, CXCL16 and EGFR by RT-qPCR; b detection of CD44, Nanog, Oct4, CXCL16 and EGFR by western blot analysis; c detection of stem cell proliferation by EdU assay; d detection of stem cell migration and invasion by Transwell assay; e tumorsphere formation assay was used to test the ability of different cells to form a sphere after transfection; f colony formation in soft agar was used to detect the ability of different cells to form colonies after transfection. The measurement data were analyzed by one-way ANOVA and expressed as mean ± standard deviation. *p < 0.05, compared with the empty vector group; the experiment was repeated 3 times