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Fig. 2 | Cancer Cell International

Fig. 2

From: LncRNA BCAR4, targeting to miR-665/STAT3 signaling, maintains cancer stem cells stemness and promotes tumorigenicity in colorectal cancer

Fig. 2

Over-expression of BCAR4 facilitated the maintenance of CSCs stemness in CRC. a HCT116 cells and HCT-8 cells were transfected with pBABE-puro-BCAR4 plasmid and pLKO.1-BCAR4 respectively. Transfection efficiency was examined by RT-PCR. b Tumorsphere formation assay revealed that BCAR4 promoted CRCs self-renewal and inhibition of BCAR4 impaired the capacity of HCT-8 cells self-renewal. c FACS assay showed HCT-116 cells transfected with pBABE-puro-BCAR4 plasmid increased the population of ALDH+ cells and inhibition of BCAR4 decreased the population of ALDH+ cells. d ALDH activity assay was used to determine the stemness alteration after BCAR4 overexpression or inhibition. e qRT-PCR results showed the expression of CSCs markers, such as NANOG, OCT4 and SOX2 in HCT116 cells with BCAR4 overexpression or inhibition. f, g Western blot was adopted to examine the expression of CSC markers after BCAR4 overexpression or inhibition. ***P < 0.001 vs. control

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