Fig. 4

BCAR4-miR-665-STAT3 ceRNA network was functioned in CRC cells. a Starbase v2.0 was used to screen the potential interaction between BCAR4 and miR-665. b RNA pull down and qRT-PCR assay showed the enrichment of miR-665. c qRT-PCR was applicable to test the expression of miR665. d Targetscan was used to predict the interaction of miR-665 and STAT3. e Luciferase reporter assay was used to examine the interaction between miR-665 and STAT3. f qRT-PCR and western blot revealed the expression of STAT3 after miR-665 overexpression g luciferase reporter assay indicated the interaction between miR-665 and STAT3 after BCAR4 inhibition. h In CRC cells, qRT-PCR and western blot showed BCAR4 inhibition impaired the expression and phosphorylation of STAT3. i In ALDH+ CRC cells, qRT-PCR and western blot indicated miR-665 was down-regulated and STAT3 expression and phosphorylation was enhanced. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control