Fig. 5

BCAR4 promoted CRC cells proliferation, migration and self-renewal through repressing miR-665. a Tumorsphere formation assay was used to examine the stemness of colon cancer cells after inhibition of STAT3. b–d Sphere formation, CCK8 and transwell migration assays were used to check the capacity of self-renewal, proliferation and migration after BCAR4 inhibition or miR-665 inhibition plus BCAR4 defect. e, f QRT-PCR and western blot showed the difference in expression of CSC markers and STAT3 after BCAR4 inhibition or loss of function of both BCAR4 and miR-665