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Fig. 3 | Cancer Cell International

Fig. 3

From: Palmitate acid promotes gastric cancer metastasis via FABP5/SP1/UCA1 pathway

Fig. 3

PA enhances GC nuclear protein levels of SP1 to elevate cellular UCA1 and promote GC metastasis. a Western blot analysis of nuclear and plasm protein levels of SP1 in MGC803 and HGC27 treated with either 2% BSA or 0.1 mM PA. b IF images represent cellular protein levels of SP1 and its distribution of MGC803 and HGC27 treated with either 2% BSA or 0.1 mM PA. c Western blot analysis of the efficiency of siRNA of SP1. d, e qRT-PCR analysis of cellular levels of UCA1 in MGC803 and HGC27 transfected with si-SP1, si-UCA1 or both and their negative controls respectively and then treated with either 2% BSA or 0.1 mM PA for 24 h. f, g The effects of transfection with si-SP1, si-UCA1 or both and their negative controls on GC cell migration and invasion treated with either 2% BSA or 0.1 mM PA (magnification: ×200). h, i Histograms show the number of migrated and invaded cells (magnification: ×200). Five random fields are selected for statistical analysis. Data are shown as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ‘NS’ means not significant

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