Fig. 4

PA facilitates GC metastasis via FABP5/SP1/UCA1 signaling. a Western blot analysis of cellular distribution of FABP5 in MGC803 and HGC27 treated with either 2% BSA or 0.1 mM PA for 24 h. b IF images represent cellular distribution of FABP5 in MGC803 and HGC27 treated with either 2% BSA or 0.1 mM PA for 24 h. c Western blot analysis of the efficiency of siRNA of FABP5. d, e Western blot analysis of nuclear protein level of SP1 in MGC803 and HGC27 treated with either 2% BSA or 0.1 mM PA for 24 h based on knockdown of FABP5. f, g The effects of transfection with si-FABP5, si-SP1 or both and their negative controls on GC cells migration and invasion treated with either 2% BSA or 0.1 mM PA (magnification: ×200). h qRT-PCR analysis of cellular levels of UCA1 in MGC803 and HGC27 transfected with si-FABP5, si-SP1 or both and their negative controls respectively and then treated with either 2% BSA or 0.1 mM PA for 24 h. i Histogram shows the number of migrated and invaded cells (magnification: ×200). Five random fields are selected for statistical analysis. Data are shown as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ‘NS’ means not significant