Table 1 Primer sequences used for real-time PCR
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
IFNβ | AGCTCCAAGAAAGGACGAACA | GCCCTGTAGGTGAGGTTGAT |
IFNγ | ACACTGCATCTTGGCTTTGC | GCTTTCAATGACTGTGCCGT |
IRF1 | GGCCGATACAAAGCAGGAGAA | GGAGTTCATGGCACAACGGA |
IRF7 | TCCAGTTGATCCGCATAAGGT | CTTCCCTATTTTCCGTGGCTG |
IRF3 | TACACTGAGGACTTGCTGGAGGT | AAGATGGTGGTCTCCTGATCC |
IRF8 | CGAGGTTACGCTGTGCTTTG | TTATGCTTGGCTCTGTGGGG |
PDL-1 | GCTCCAAAGGACTTGTACGTG | TGATCTGAAGGGCAGCATTTC |
CXCL9 | GGAGTTCGAGGAACCCTAGTG | GGGATTTGTAGTGGATCGTGC |
CXCL10 | CCAAGTGCTGCCGTCATTTTC | GGCTCGCAGGGATGATTTCAA |
Gapdh | AGGTCGGTGTGAACGGATTTG | GGGGTCGTTGATGGCAACA |