Fig. 2

TMED3 was expectedly elevated in breast cancer cell lines. a Basal expression of TMED3 in breast cancer cell lines MDA-MB-231 and MCF-7 as well as the normal human breast mammary epithelial cell line MCF-10A and the transformed but not malignant human mammary epithelial cell line HBL-100, as detected by western-blot; b silencing effect of siRNA sequences targeting TMED3 was evaluated by western-blot in MCF-7 cell line; c likewise, the transient knock-down efficiency was evaluated in parallel in MDA-MB-231 cell line; d stable knock-down efficiency of lenti-viral based short hairpin RNA (shRNA) targeting TMED was detected using western-blot in both MCF-7 and MDA-MB-231 cell lines. All detections were done independently three times and shown were the representative figures selected among candidates. Quantitative analysis was carried out using Image J software (NIH, Bethesda, USA)