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Fig. 6 | Cancer Cell International

Fig. 6

From: Silencing of SNHG6 induced cell autophagy by targeting miR-26a-5p/ULK1 signaling pathway in human osteosarcoma

Fig. 6

miR-26a-5p was an inhibitory target of SNHG6. a The pGL3 Basic Luciferase Reporter Vectors. b Sequence alignment of miR-26a-5p with the putative binding sites with in the wild-type regions of SNHG6. Dual-luciferase reporter assay showed that an obviously reduction of luciferase activity in 293T cells of SNHG6-Wt transfection, while no effect on the SNHG6-mut vector. c qRT-PCR analysis of miR-26a-5p expression in OS cells transfected with siRNA-SNHG6 or siRNA-NC. d qRT-PCR analysis of SNHG6 expression in OS cells transfected with miR-26a-5p or miR-NC. All samples were assayed in triplicate. *P < 0.05

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