Fig. 4
From: The Hippo signaling effector WWTR1 is a metastatic biomarker of gastric cardia adenocarcinoma
WWTR1 is required for gastric cancer AGS cell migration. The transwell assay is used to determine the effect of WWTR1 knockdown on AGS cell migration. The gastric cancer cell migration assays were repeated three times. For quantification of the migration rate in the transwell assay, the migrated cells (bottom side of the membrane) were fixed and stained by the crystal violet. The cells were counted under a microscope from three randomly selected fields. a The effect of shWWTR1-1; b the effect of shWWTR1-2. EGF (50 ng/ml) was used for stimulation of cell migration. ***p < 0.001