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Fig. 2 | Cancer Cell International

Fig. 2

From: Overexpression and Tyr421-phosphorylation of cortactin is induced by three-dimensional spheroid culturing and contributes to migration and invasion of pancreatic ductal adenocarcinoma (PDAC) cells

Fig. 2

Characterization of cortactin expression and subcellular localization in PDAC cell lines and establishment of cortactin knockdown mediated by CRISPR/Cas9 technology. a Immunoblotting of whole cell lysates of PDAC cell lines for cortactin and Src and their activated forms (p-cortactin Tyr421 and p-Src Tyr416). The MCF-7 breast carcinoma cell line was included as positive control. b Cortactin localization (green) in BxPC-3 and Panc-1. The nucleus was counterstained with DAPI (blue). Arrowheads indicate cortactin expression at sites of cell–cell-contacts. Scale bar, 50 µm. c Serum starved Panc-1 cells were stimulated with 10% FCS for 1 h. After stimulation, cortactin (green) localized to the cell membrane. The actin cytoskeleton was stained with phalloidin (red) and the nucleus was counterstained with DAPI (blue). Scale bar, 50 µm. d Immunoblotting of CRISPR/Cas9 mediated cortactin knockdown (CTTN KO) cells. Cells were grown as single selected clones. GAPDH was used as loading control

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Cancer Cell International

ISSN: 1475-2867

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