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Fig. 3 | Cancer Cell International

Fig. 3

From: Overexpression and Tyr421-phosphorylation of cortactin is induced by three-dimensional spheroid culturing and contributes to migration and invasion of pancreatic ductal adenocarcinoma (PDAC) cells

Fig. 3

Functional analysis of cortactin knockdown (CTTN KO) cells and controls (CTRL). a, c Knockdown of cortactin did not affect pancreatic cell proliferation measured with the xCELLigence system of BxPC-3 and Panc-1 cells. b, d Cell migration assessed in vitro performing scratch assays showed significantly impaired migration of CTTN KO cells compared to the controls. e Cell migration stimulated with 0–2.5% FCS was measured with the xCELLigence system and showed significantly reduced migration in Panc-1 CTTN KO cells. Bar chart indicates the relative cell migration normalized to control cells after 8 h. f Quantification of matrix degradation. No significant differences were observed between BxPC-3 CTTN KO cells and controls. g BxPC-3 cells were stained with DiO prior to spheroid culture for collagen cell invasion assays. Spheroids cultured for 24 h were then embedded in a collagen suspension and cell invasion was assessed 12 and 24 h after plating. BxPC-3 CTTN KO cells showed significantly impaired invasion compared to the controls. Scale bar, 1000 µm. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant. Data points shown are the mean + SD. The results are representative of at least three independent experiments

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