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Fig. 5 | Cancer Cell International

Fig. 5

From: Overexpression and Tyr421-phosphorylation of cortactin is induced by three-dimensional spheroid culturing and contributes to migration and invasion of pancreatic ductal adenocarcinoma (PDAC) cells

Fig. 5

Gelsolin is a novel interaction partner of cortactin in PDAC. a, b Verification of mass spectrometry results by Co-immunoprecipitation (Co-IP) of gelsolin by cortactin antibody. Co-IP with cortactin antibody showed cortactin precipitation and co-precipitation of gelsolin in a BxPC-3 and b Panc-1 cells. CTTN KO cells served as negative controls. Black arrows indicate size of gelsolin and cortactin. c Immunofluorescence staining of gelsolin (green) and cortactin (red) confirmed the possible interaction as co-localization of both proteins could be detected. Scale bar, 50 µm. d In cells cultured in spheroids upregulation of gelsolin expression compared to cells in adherent culture was detected. GAPDH was used as loading control. e Scheme of the role of cortactin in pancreatic cancer migration. ab, antibody; adh, adherent; IB, immunoblot; IP, Immunoprecipitation; sph, spheroid; WCL, whole cell lysate; Wt, wild type

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Cancer Cell International

ISSN: 1475-2867

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