Table 1 Primer sequence for RT-qPCR
Gene | Primer sequence (5′–3′) |
|---|---|
LINC01314 | F: TGGGGCTGCCCTGGCCCGAGGGAC |
R: CCAGTGCAGGGTCCGAGGT | |
KLK4 | F: ATCTGCCTACCTCCGGAGTC |
R: TTAACTGTCCTGGATGGTTTT | |
Wnt-1 | F: GCTAGCGAAAGTCATTGGC |
R: CATTGCATCGAAGTCAGTG | |
β-catenin | F: GCTAGCTAAGTCAGTCGGG |
R: CGTATATAGCTAGCTGGCTA | |
cyclin D1 | F: AAGCCTCAGCCCTCCCCAGCTGCCAG |
R: AACCAACAACAAGGAGGATG | |
N-cadherin | F: TTTGATGGAGGTCTCCTAACACC R: ACGTTTAACACGTTGGAAATGTG |
E-cadherin | F: CGAGAGCTACACGTTCACGG |
R: GGGTGTCGAGGGAAAAATAGG | |
GAPDH | F: CGGAGTCAACGGATTTGGTAT |
R: AGCCTTCTCCATGGTGGTGAAGAC |