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Fig. 2 | Cancer Cell International

Fig. 2

From: PITX2 enhances progression of lung adenocarcinoma by transcriptionally regulating WNT3A and activating Wnt/β-catenin signaling pathway

Fig. 2

Knockdown of PITX2 suppressed malignant phenotype of lung adenocarcinoma in vitro. a Two siRNAs effectively knocked down the expression of PITX2 in NCIH1299 cell lines (interfering efficiency, si1 75.3%; si2 75.29%). b, c CCK-8 and RTCA assays showed that knockdown of PITX2 inhibited proliferation of NCIH1299 cell lines. d In colony formation assay, NCIH1299 cell lines transfected with si-PITX2 presented less colony count compared with control group (si1, p < 0.001; si2, p < 0.001). e, f Confirmed by transwell and matrigel assays, knockdown of PITX2 suppressed the migration and invasion ability of NCIH1299 cell lines (si1, p < 0.001; si2, p < 0.001). g Knockdown of PITX2 induced G1/S cell cycle arrest in NCIH1299 cell lines (si1, p < 0.001; si2, p < 0.001). h TUNEL assay revealed that NCIH1299 cell lines exerted higher apoptosis ratio after knockdown of PITX2 (si1, p < 0.001; si2, p < 0.001) (*p < 0.05, **p < 0.01, ***p < 0.001)

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