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Fig. 3 | Cancer Cell International

Fig. 3

From: PITX2 enhances progression of lung adenocarcinoma by transcriptionally regulating WNT3A and activating Wnt/β-catenin signaling pathway

Fig. 3

PITX2 promoted aggressiveness of lung adenocarcinoma via regulating Wnt/β-catenin signaling pathway. a, b Gene_Ontology and Reactome Pathway analysis showed that PITX2 might participate in “signal transduction”, “apoptotic process”, “regulation of proliferation” and “gene transcription”. c In String Database, PITX2 is predicted to interact with LEF1, CTNNB1 and AXIN, all of which are the key factors of Wnt/β-catenin signaling pathway. d, e QRT-PCR and western blotting indicated that knockdown of PITX2 decreased the expression of Wnt/β-catenin downstream genes (CCND1, CD44, MYC, JUN) in LUAD cells. f Silencing of PITX2 reduced the transcriptional activity of β-catenin while Wnt agonist1, an activator of Wnt/β-catenin pathway, restored the effect. g Wnt agonist1 rescued the influence of PITX2 on proliferation in LUAD cells. h In TUNEL assay, Wnt agonist1 reversed the increased apoptosis caused by knockdown of PITX2 in LUAD cells (*p < 0.05, **p < 0.01, ***p < 0.001)

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