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Fig. 2 | Cancer Cell International

Fig. 2

From: RNF8 is responsible for ATRA resistance in variant acute promyelocytic leukemia with GTF2I/RARA fusion, and inhibition of the ubiquitin–proteasome pathway contributes to the reversion of ATRA resistance

Fig. 2

Recruitment of transcriptional corepressors by GTF2I-RARA in 293T cells. 293T cells were transfected with a FLAG-tagged GTF2I-RARA expression plasmid. a Coimmunoprecipitation between endogenous corepressors and GTF2I-RARA, which interacted with HDAC3, N-CoR, and SMRT. Input, non-immunoprecipitated cell lysates; IgG, control IP with isotype antibody. b Coimmunoprecipitation between endogenous corepressors and GTF2I-RARA in the presence of ATRA. GTF2I-RARA did not dissociate with HDAC3 but no longer interacted with SMRT and N-CoR. c Immunofluorescent colocalization of FLAG-tagged-GTF2I-RARA and corepressors with or without ATRA. 4′,6-diamidino-2-phenylindole (DAPI) was used for nuclear staining. At least 100 cells were counted for colocalization analysis. Scale bar = 8 μm

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