New therapeutic aspects of steroidal cardiac glycosides: the anticancer properties of Huachansu and its main active constituent Bufalin

Cheng, Chien-shan; Wang, Jiaqiang; Chen, Jie; Kuo, Kuei Ting; Tang, Jian; Gao, Huifeng; Chen, Lianyu; Chen, Zhen; Meng, Zhiqiang

doi:10.1186/s12935-019-0806-1

Table 1 Growth inhibition of Bufalin (BF) on human cancer cell lines after 24–72H treatment

From: New therapeutic aspects of steroidal cardiac glycosides: the anticancer properties of Huachansu and its main active constituent Bufalin

Cancer type	Cell line	24H IC50	48H IC50	72H IC50	Refs.
Breast	MCF-7	317.9 ± 1.5 nM	46.5 ± 1.4 nM		Yan et al. [125]
	MCF-7	3.2 nM			Wang et al. [56]
	MCF-7/ADR		100.17 ± 30.16 nmol/L		Wang et al. [126]
	MCF-10A	465.2 ± 25.9 nM			Clifford et al. [31]
	MCF10CA1	635.2 ± 171.8 nM			Clifford et al. [31]
	MDA-MB-231	934.1 ± 2.0 nM	513.3 ± 1.6 nM		Yan et al. [125]
			263.3 ± 68.24 nmol/L		Wang et al. [126]
		936.4 ± 4.9 nM			Clifford et al. [31]
				20.0 nM	Song et al. [55]
	MDA-MB-231-LM3-3			16.6 nM	Song et al. [55]
	SUM149PT			16.6 nM	Song et al. [55]
	SUM159PT			15.9 nM	Song et al. [55]
Cervical	Hela	154 ± 21.5 nM	37.5 ± 2.15 nM	11.8 ± 2.13 nM	Pan et al. [127]
Colorectal	SW620	76.72 ± 6.21 nmol/L	34.05 ± 4.21 nmol/L	16.7 ± 6.37 nmol/L	Zhu et al. [128]
	SW620	287.35 ± 4.34 nM	57.63 ± 7.80 nM	20.39 ± 1.95 nM	Zhang et al. [129]
	HCT116	82.6 μmol/L			Qiu et al. [130]
	HCT116	0.243 μM	0.024 μM		Wang et al. [131]
	LoVo	56.778 ± 7.34 nM	11.48 ± 2.89 nM	6.64 ± 2.79 nM	Zhang et al. [129]
Endometrial	Ishikawa		0.7 ng/mL		Takai et al. [132]
	HHUA		0.5 ng/mL		Takai et al. [132]
	HEC-1B		0.6 ng/mL		Takai et al. [132]
Esophageal	Eca-109			1.0 μM	Lv et al. [133]
	EC9706			1.0 μM	Lv et al. [133]
	TE5			2.6 μM	Lv et al. [133]
	TE11			4.9 μM	Lv et al. [133]
	Hec2			3.8 μM	Lv et al. [133]
Gallbladder	GBC-SD		48.12 ± 2.03 nM	28.23 ± 1.78 nM	Jiang et al. [45]
Gallbladder	SGC996		125.03 ± 5.16 nM	102.78 ± 3.21 nM	Jiang et al. [45]
Gastric	SGC7901	> 800 nmol/L			Li et al. [134]
Gastric	MGC803	160 ± 0.87 nmol/L			Li et al. [134]
Liver	HepG2	182.30 ± 13.78 nM			Li et al. [27]
		307 ± 87 nM			Ozdemir et al. [84]
			33.65 nmol/L		Gao et al. [60]
			143.2 nM		Miao et al. [49]
	PLC/PRF/5	52.20 ± 14.16 nM			Li et al. [27]
	PLC/PRF/5		157.87 nmol/L		Gao et al. [60]
	SMMC7721	97.74 ± 8.83 nM			Li et al. [27]
	SK-HEP-1	110.33 ± 5.32 nM			Tsai et al. [135]
Leukemia	NB4	40 nmol/L	27 nmol/L	17 nmol/L	Zhai et al. [136]
	K562			0.0943 μmol/L	Zhai et al. [136]
	K562/VCR			0.0401 μmol/L	Zhai et al. [136]
	HEL		0.046 μmol/L		Wang et al. [137]
Lung	A549	4.5 nM			Wang et al. [56]
			56.14 ± 6.72 nmol/L	15.57 ± 4.28 nmol/L	Zhu et al. [138]
				8.15 ± 0.69 nmol/L	Sun et al. [92]
		22.00 ± 3.53 nM	10.20 ± 1.01 nM		Liu et al. [36]
Melanoma	A375.S2		450.38 nM		Hsiao et al. [44]
Oral	CAL27	125 nM			Tsai et al. [139]
Oral	CAL27	122.6 nM			Tian et al. [140]
Osteosarcoma	U-2OS	0.297 μM			Zhang et al. [141]
	U-2OS		8.49 ± 2.1 μg/L		Yin et al. [142]
	U-2OS/MTX300		10.19 ± 1.7 μg/L		Yin et al. [142]
	Saos-2	0.318 μΜ			Zhang et al. [141]
Ovarian	SK-OV-3		1.0 ng/mL		Takai et al. [132]
Ovarian	OMC-3		0.6 ng/mL		Takai et al. [132]
Tongue	SCC-4		300 nM		Chou et al. [143]
Pancreatic	Capan-2	159.2 nM			Tian et al. [140]
Non-cancerous	Breast epithelial cell 184D	384.6 ± 36.5 nM			Clifford et al. [31]
	Breast epithelial cell 184A	295.9 ± 10.5 nM			Clifford et al. [31]
	Mouse normal hepatocyte			No cytotoxicity at 10 μM	Song et al. [55]
	HUVECs	53.1 nmol/L			Qiu et al. [130]
	Het-1A^a			10.1 μM	Lv et al. [133]
	PBMCs^b		16.5 ± 4.7 ng/mL		Yuan et al. [144]
	Primary normal human endometrial epithelial cells		Little sensitivity to BF from 0.1 to 10 ng/mL		Takai et al. [132]
	PBMCs		16.5 ± 4.7 ng/mL		Yuan et al. [144]

^aHuman normal esophageal squamous cells
^bNormal peripheral blood mononuclear cells (PBMCs) were isolated from three healthy volunteers (32 ± 9 years of age)

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