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Fig. 3 | Cancer Cell International

Fig. 3

From: Silencing long non-coding RNA HOTAIR exerts anti-oncogenic effect on human acute myeloid leukemia via demethylation of HOXA5 by inhibiting Dnmt3b

Fig. 3

Silencing HOTAIR upregulates HOXA5 to promote apoptosis and suppress proliferation of HL-60 cells. a Expression of HOTAIR in HL-60 cells after interference with HOTAIR or HOXA5 detected by RT-qPCR. b Expression of HOXA5 at mRNA level in HL-60 cells after interference with HOTAIR or HOXA5 examined by RT-qPCR. c Protein expression of HOXA5 in HL-60 cells after interference with HOTAIR or HOXA5 detected by western blot analysis. d Expressions of cyclin G, Bax, Bcl-2, MCP-1, cleaved-caspase3, p27 and cyclin G in HL-60 cells after interference with HOTAIR or HOXA5 examined by western blot analysis. e Cell apoptosis in HL-60 cells after interference with HOTAIR or HOXA5 examined by flow cytometry. f Cell cycle distribution of HL-60 cells after interference with HOTAIR or HOXA5 detected by flow cytometry. g Proliferation of HL-60 cells after interference with HOTAIR or HOXA5 examined by EdU assay (×200). *p < 0.05 vs. the sh-NC group; #p < 0.05 vs. the sh-HOTAIR + sh-NC group. RT-qPCR reverse transcription quantitative polymerase chain reaction, HOTAIR Hox transcript antisense intergenic RNA, HOXA5 homeobox A5, EDU 5-ethynyl-2′-deoxyuridine, AML acute myeloid leukemia, Bcl-2 B-cell lymphoma 2, Bax Bcl-2 associated X, MCP-1 monocyte chemoattractant protein 1, NC negative control. The results were measurement data. Comparisons among multiple groups were assessed by one-way analysis of variance. The experiment was independently repeated three times

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Cancer Cell International

ISSN: 1475-2867

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