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Fig. 5 | Cancer Cell International

Fig. 5

From: Silencing long non-coding RNA HOTAIR exerts anti-oncogenic effect on human acute myeloid leukemia via demethylation of HOXA5 by inhibiting Dnmt3b

Fig. 5

Silenced HOTAIR enhances apoptosis and inhibits proliferation of HL-60 cells in vivo by upregulating HOXA5. a The volume of tumor from nude mice transplanted with HL-60 cells overexpressing HOTAIR or HOXA5. b Expression of Bax, Bcl-2, MCP-1, cleaved-caspase3, p27 and cyclin G in HL-60 cell overexpressing HOTAIR or HOXA5 detected by western blot analysis. c EDU assay to evaluate the proliferation of HL-60 cells after overexpressing HOTAIR or HOXA5 (×200). *p < 0.05 vs. the M-oe-NC and M-sh-NC groups; #p < 0.05 vs. the M-oe-HOTAIR and M-sh-HOTAIR groups. HOTAIR Hox transcript antisense intergenic RNA, HOXA5 homeobox A5, EDU 5-ethynyl-2′-deoxyuridine, Bcl-2 B-cell lymphoma 2, Bax Bcl-2 associated X, MCP-1 monocyte chemoattractant protein 1, NCnegative control. The results were measurement data. Comparisons among multiple groups were assessed by one-way analysis of variance. The experiment was independently repeated three times

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