Fig. 4

Knockdown of PGC-1β combined with overexpression of FOXA2 inhibit proliferation and migration of breast cancer cells. MCF-7 and MDA-MB-231 cells were transfected with overexpression plasmid (FOXA2) and interference plasmid (sh-PGC-1β) to detect the cell proliferation and migration. a Cell growth was evaluated by CCK-8 assay. b In vitro proliferative ability of two cell lines were detected by clone formation assay. c Cell metastatic was assessed using Transwell assay. (MCF-7: original magnification ×200, scale bar = 50 μm; MDA-MB-231: original magnification ×40, scale bar = 200 μm). d In vitro migration ability were detected using scratch wound healing assay (scale bar = 200 μm). *P < 0.05, **P < 0.01. ***P < 0.001