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Fig. 3 | Cancer Cell International

Fig. 3

From: Large tumor suppressor kinase 2 overexpression attenuates 5-FU-resistance in colorectal cancer via activating the JNK-MIEF1-mitochondrial division pathway

Fig. 3

Mitochondrial dysfunction is triggered by 5-FU treatment and this effect is further augmented by LATS2 overexpression. a, b Mitochondrial membrane potential was determined via JC-1 probe. Red fluorescence means the normal mitochondrial membrane potential whereas green fluorescence indicates the damaged mitochondrial potential. The red-to-green fluorescence ratio was used to quantify mitochondrial membrane potential. c Cellular ATP production was determined. LATS2 adenovirus vectors (ad-LATS2) were transfected into SW480 cells. dg Proteins were isolated from SW480 cells and then the expression of mitochondrial respiratory complex was determined via western blotting. *p < 0.05 vs. control (ctrl) group; #p < 0.05 vs. 5-FU + ad-ctrl group; ad-ctrl: control null adenovirus vectors; ad-LATS2: LATS2 adenovirus vectors

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