Fig. 1

OTUD4 is downregulated and associates with poor prognosis in NSCLC. Western blotting (a) and real-time PCR (b) analysis of the expression of OTUD4 in NSCLC cell lines. Western blotting (c) and real-time PCR (d) analysis of the expression of OTUD4 in paired adjacent non-tumor (NT) and tumor (T) tissues from NSCLC patients. e Expression level of OTUD4 in normal and tumor tissues of patients from the TCGA dataset. Error bars represent Median with interquartile range. N, normal. LUAD, lung adenocarcinoma. LUSC, lung squamous cell carcinoma. f Expression level of OTUD4 in normal (N) and tumor (T) tissues of patients from the GEO/GSE2514 dataset. Error bars represent Median with interquartile range. Kaplan–Meier analysis of the OS (g), FPS (h) and PPS (i) in lung cancer using Kaplan–Meier Plotter (http://kmplot.com/analysis/). OS, overall survival. FP, first progression. PPS, post progression survival. Tubulin served as loading control. *p < 0.05