Fig. 4

TCF4 is as target of miR-591 in breast cancer. a The miR-591-binding sites in the 3′-UTR of TCF4 was shown. b MCF-7 cells were co-transfected with pMIR-REPORT-TCF4-3′UTR WT or pMIR-REPORT-TCF4-3′UTR MUT, together with miR-591 mimic or miR-NC. Luciferase activity was measured at 48 h after transfection. c, d TCF4 mRNA expression in MCF-7 or SKBR3 cells transfected with miR-591 mimic, miR-591 inhibitor or miR-NC was measured using qRT-PCR. e, f TCF4 protein expression in MCF-7 or SKBR3 cells transfected with miR-591 mimic or miR-NC was measured using western blot analysis. All the data are expressed as the mean ± SD. *P < 0.05