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Fig. 4 | Cancer Cell International

Fig. 4

From: Hypoxia promotes colorectal cancer cell migration and invasion in a SIRT1-dependent manner

Fig. 4

EGR1 bound directly to GC-rich recognition motifs of the SIRT1 promoter. a Dual-luciferase reporter system analysis of the transcriptional activities of EGR1 for all truncated segments of the SIRT1 promoter region. Each group was analyzed in triplicate, and each experiment was performed three times. b Dual-luciferase reporter system analysis of the transcriptional activities of increasing EGR1 concentrations for the − 100 ~ + 66 bp segments of the SIRT1 promoter region. Each group was analyzed in triplicate, and each experiment was performed three times. c A ChIP-PCR assay demonstrated that EGR1 binds directly to − 100 ~ + 66 bp of the SIRT1 promoter region under normoxia and disassociates from this segment under hypoxia. d Schematic diagram indicating the EGR1-binding GC-rich motifs and their mutated sequences within 100 bp nucleotides upstream of the SIRT1 promoter sequence. The TATA box is also indicated. e Dual-luciferase reporter system analysis of the transcriptional activities of EGR1 for the control and mutated promoter sequences. ***p < 0.001

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