Fig. 5

EGR1 reversed hypoxia-induced CRC cell migration and invasion. a An EGR1 overexpression plasmid and its empty vector were transiently transfected into HCT116 and SW480 cells, and then subjected to normoxia and hypoxia for 24 h, respectively. Cell lysates were analyzed by Western blot to detect the expression levels of EGR1 and SIRT1. Scanning densitometry of immunoblotting for each panel was measured (right). b An EGR1 overexpression plasmid and its empty vector were transiently transfected into HCT116 cells for 24 h. Transwell chambers were then used to detect the migration and invasion abilities of the HCT116 cells after exposure to normoxia or hypoxia for another 24 h. c Column diagram indicating the migrating and invading cell counts per high power microscope field and the statistical analysis results. Five random high power fields were counted for each group. ***p < 0.001