Fig. 6

SIRT1 modulated the NF-κB pathway in CRC cells under hypoxic conditions. a Western blot analysis of the NF-κB p65 acetyl lysine 310 residue, NF-κB p65, MMP-2, MMP-9 and TIMP-2 in HCT116 and SW480 cells under hypoxic conditions. Scanning densitometry of immunoblotting for each panel was measured (right). b Real-time PCR analysis of MMP-2, MMP-9, TIMP-2 and TIMP-3 in CRC cells under hypoxic conditions. c Western blot analysis of NF-κB p65 and its acetyl lysine 310 residue in SIRT1 knockdown and SIRT1 overexpression CRC cells. Scanning densitometry of immunoblotting for each panel was measured (right). d Schematic model indicating the intracellular bioprocesses regulating SIRT1 transcription and CRC cell migration and invasion under normoxia and hypoxia