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Fig. 4 | Cancer Cell International

Fig. 4

From: Emodin inhibits colon cancer by altering BCL-2 family proteins and cell survival pathways

Fig. 4

Effect of Emodin on expression and localization of BCL-2 family proteins. Heat map showing Emodin induced changes in Bcl-2 family apoptotic proteins was generated from the apoptosis antibody microarray data (a). Red represents increase while green represents decrease in protein expression, color intensity indicates on level of expression. Effect of Emodin treatment (DLD-1 ~ 18 μM; COLO 201 ~ 15 μM; 36 and 48h) on Bad, Bak, Bax, Bid, Bim, Bcl-2, and PUMA expression compared to vehicle control is shown by western blot (b). β-actin was used as a loading control. The quantitative bar graphs shown represent ratio of protein in Emodin treated versus vehicle-treated after normalization with β-actin band intensity. Effect of Emodin on cytosolic (c) and mitochondrial (d) distribution of  BAX, Bcl-2 and Cytochrome C is shown by western blot. Bar graphs show the quantitative distribution of the proteins in the two cellular fractions. β-actin or GAPDH was used as the loading control for cytosolic fractions and Hsp70 was used as a loading control for mitochondrial fractions. Densitometry analysis was done with respect to the intensities of loading control. All graphs represent the mean fold change intensity value ± SEM from three independent experiments; Asterisks (*) p < 0.05, (**) p < 0.01, (***) p < 0.001 show significant change after Emodin treatment compared with the control

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