Fig. 1

Expression of USP6NL in human CRC. a mRNA level of USP6NL in 32 pairs of tumorous colorectal tissues and adjacent non-tumorous tissue, detected using RT-PCT. b mRNA level of USP6NL in CRC patients from The Cancer Genome Atlas (TCGA) dataset (n = 260) and corresponding healthy people (n = 41). c mRNA level of USP6NL in CRC patients from GEO dataset (n = 70) and corresponding healthy people (n = 12). d IHC staining showed that USP6NL was up-regulated in tumor tissue (n = 10) when compared with adjacent-precancerous tissues (n = 5) from CRC patients (original magnification ×200). e mRNA and protein levels of USP6NL in five CRC cell lines (HT29, SW480, LOVO, HCT116 and CACO2) and one human normal colon FHC cells were assessed, using RT-PCR and western blot method, respectively. ^##P < 0.01 vs. precancerous tissue; ^$$P < 0.01 vs. Healthy people; **P < 0.01 vs. FHC cells