Fig. 2
From: Tre2 (USP6NL) promotes colorectal cancer cell proliferation via Wnt/β-catenin pathway
Knockdown of USP6NL inhibited CRC cell growth and prevented Wnt/β-catenin pathway activation. a, e mRNA and protein levels of USP6NL, assessed by RT-PCR and western blot, respectively, were significantly down-regulated in HCT116 and LOVO cells, suggesting a successful establishment of USP6NL silencing within those two CRC cell lines. b, f CCK-8 analysis showed that siUSP6NL (siRNA-USP6NL-1) significantly inhibited the proliferation of HCT116 and LOVO cells. c, g Flow cytometry showed that siUSP6NL obviously enhanced the proportion of HCT116 and LOVO cells in G0–G1 while reduced cell rates in S and G2 phases. f, h Western blot analysis showed that siUSP6NL remarkably down-regulated β-catenin, Cyclin D1 and C-myc while up-regulated P27 in HCT116 and LOVO cells. **P < 0.01 vs. siNC