Fig. 5

Effect of intervention of Nectin-4 expression on cell proliferation of human EC cell lines. We examined the intervention of Nectin-4 expression on the cell proliferation in vitro using CCK-8 assay in human EC cell lines. a In Eca-109 cells, at 48 and 72 h after seeding, the cell proliferation rate of LV-Nectin-4-shRNA group was significantly lower compared with the LV-NC group (both P < 0.01). b In Eca-109 cells, at 48 and 72 h after seeding, the cell proliferation rate of LV-Nectin-4-OE group was significantly higher compared with the LV-Vector-Ctrl group (both P < 0.01). c In TE-1 cells, at 24, 48 and 72 h after seeding, the cell proliferation rate of LV-Nectin-4-shRNA group was significantly lower compared with the LV-NC group (all P < 0.01). d In TE-1 cells, at 48 and 72 h after seeding, the cell proliferation rate of LV-Nectin-4-OE group was significantly higher compared with the LV-Vector-Ctrl group (both P < 0.01)