Fig. 7

The intervention of Nectin-4 expression affects the invasion ability of human EC cell lines. The transwell invasion assay was performed to evaluate the effect of the intervention of Nectin-4 expression on the invasion ability of human EC cell lines. a Knockdown of Nectin-4 expression significantly decreased the number of invaded cells stained with Cristal Violet in LV-Nectin-4-shRNA group cells compared with LV-NC group cells (in Eca-109, P < 0.01, and in TE-1, P < 0.001). b Nectin-4 over-expression significantly increased the number of invaded cells stained with Cristal Violet in LV-Nectin-4-OE group cells compared with LV-Vector-Ctrl group cells (both P < 0.001)