Fig. 8

Intervention of Nectin-4 expression effects the tumor growth in the subcutaneous transplantation mouse model. The subcutaneous transplantation mouse model was used to evaluate the effect of the intervention of Nectin-4 expression on the tumor growth of human EC cell lines. The Eca-109 or TE-1 cells from LV-NC, LV-Nectin-4-shRNA, LV-Vector-Ctrl and LV-Nectin-4-OE groups were injected subcutaneously into nude mice (n = 5, respectively). a In tumor models established by using Eca-109 cells, the tumor growth curves showed that knockdown of Nectin-4 expression significantly inhibits tumor growth (P < 0.0001), and the Nectin-4 over-expression significantly promotes tumor growth (P < 0.0001). b The tumor tissues resected from the tumor models established by Eca-109 cells from each groups were listed. c After 28 days, in tumor models established by using Eca-109 cells, the tumor weight of LV-Nectin-4-shRNA group was lighter than that of LV-NC group (P < 0.05), the tumor weight of LV-Nectin-4-OE group trended heavier than that of LV-Vector-Ctrl group (P < 0.001). d In tumor models established by using TE-1 cells, the tumor growth curves showed that knockdown of Nectin-4 expression significantly inhibits tumor growth (P < 0.0001), and the Nectin-4 over-expression significantly promotes tumor growth (P < 0.001). e The tumor tissues resected from the tumor models established by TE-1 cells from each groups were listed. f After 28 days, in tumor models established by using TE-1 cells, the tumor weight of LV-Nectin-4-shRNA group was lighter than that of LV-NC group (P < 0.0001), the tumor weight of LV-Nectin-4-OE group trended heavier than that of LV-Vector-Ctrl group (P = 0.1314)