Fig. 2

Different concentrations of dUTP had similar expression levels of marker genes in pediatric ALL. There was no significant disparity in the efficiency of amplification among the three panels. Hence, we chose only Panel 2 to determine the concentration of dUTP. a–d represent dUTP:dTTP = 0:3; 1:3; 2:3 and 3:3, respectively. a 3.5 mM dTTP; b 0.875 mM dUTP and 2.625 mM dTTP; c 1.4 mM dUTP and 2.1 mM dTTP; d 1.75 mM dUTP and 1.75 mM dTTP