Fig. 2

CHIP affects the migration and invasion abilities of AGS cells. a The migration ability of the cells was detected by the scratch healing assay at 24 h and 48 h. b Representative images of the Transwell migration assay. The numbers of migrated cells were calculated. Cells were fixed, stained, and photographed. Bar chart represents mean ± SD. All images were representative of at least three independent experiments with similar findings. c The migration curves of the sictrl and siCHIP cells were detected using the real-time x-Celligence system. d Representative images of the Transwell invasion assay. The sictrl and siCHIP cells were allowed to migrate through the Transwell inserts pre-coated with Matrigel (1:40 dilutions) for 24 h. The numbers of invasive cells were calculated. Cells were fixed, stained, and photographed. Each bar represents mean ± SD. All images were representative of at least three independent experiments with similar findings. e The invasion ability of the two established cell lines was detected by the real-time xCelligence monitoring system using Matrigel (1:40 dilutions)-coated CIM-plates. f Western blotting analysis of protein expression of Integrin-β1, uPA, MMP2, and MMP9. Actin was used as an internal control