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Fig. 4 | Cancer Cell International

Fig. 4

From: The ubiquitin ligase CHIP modulates cellular behaviors of gastric cancer cells by regulating TRAF2

Fig. 4

TRAF2-silencing inhibited the cell growth of AGS cells. a The cell growth curves of the sictrl and siTRAF2 cells were detected using the x-Celligence system. E-plate was seeded with 4000 cells/well and cell growth was continuously monitored for 72 h. b Cell proliferation of the siTRAF2 and sictrl cells was determined by the CCK-8 assay. 96-well plate was seeded with 5000 cells/well. OD450 was measured by means of spectrophotometer microplate reader. c The CFSE proliferation assay was examined by flow cytometry analysis at 24, 48 and 72 h. d Cell viability assay. 96-well plate was plated with 4000 cells/well. OD570 was measured by means of spectrophotometer microplate reader at 24, 48 and 72 h. e The apoptosis of the sictrl and siTRAF2 cells was determined by the Annexin V/PI staining. The bar chart represents the percentages of apoptotic cells of the two established cell lines. The mean ± SD from three individual experiments were displayed

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