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Fig. 5 | Cancer Cell International

Fig. 5

From: The ubiquitin ligase CHIP modulates cellular behaviors of gastric cancer cells by regulating TRAF2

Fig. 5

TRAF2-silencing inhibited the migration and invasion of AGS cells. a The migration ability of the cells was detected by the scratch healing assay at 24 h and 48 h. b Representative images of the Transwell migration assay. The numbers of migrated cells were calculated. Cells were fixed, stained, and photographed. Bar chart represents mean ± SD. All images were representative of three independent experiments. c The migration abilities of the sictrl and siTRAF2 cells were detected using the real-time x-Celligence system. d Representative images of the Transwell invasion assay. The sictrl and siTRAF2 cells were allowed to migrate through the Transwell inserts pre-coated with Matrigel (1:40 dilutions) for 24 h. The numbers of invasive cells were calculated. Cells were fixed, stained, and photographed. Each bar represents mean ± SD. All images were representative of at least three independent experiments. e The invasion ability of the sictrl and siTRAF2 cell lines was detected by the real-time xCelligence system using Matrigel (1:40 dilutions)-coated CIM-plates

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