Table 2 Summary of HXEX-ALL1 cell features
Feature | HXEX-ALL1 cells |
|---|---|
Clinical characteristics | |
 Patient | 6-year-old male |
 Diagnosis | BCP-ALL |
 Treatment status | At second relapse |
 Specimen site | Bone marrow |
 Year of establishment | 2017 |
Cell culture characteristics | |
 Culture medium | 90% RPMI-1640 + 10% FBS |
 Growth pattern | Suspension as single cells or clumps |
 Doubling time | 26–32 h |
 Subculture | 1:4 |
 Maximum cell density | 10 × 106 cells/ml |
 Optimal cell density | 1 ~ 3×106 cells/ml |
 Cryoperservation | 60% RPMI-1640 + 30% FBS + 10% DMSO |
 Morphology | Medium-sized spheroid morphology |
 Viral status | Negative for EBV, HCMV, HBV, and HIV |
 Contamination | Negative for mycoplasma |
 Authentication | Yes (by STR analysis, cytogenetic analysis, and immunoprofiling) |
Immunophenotypic characteristics | |
 B cell | CD10+, CD19+, CD22+, cCD79α+, CD20−, cIgM−, sIgM− |
 T cell | CD2−, CD3−, CD5+, CD7−, cCD3− |
 Myelocytic | CD13−, CD33−, CD117− |
 Progenitor | CD34−, HLA-DR+ |
Ig/TCR rearrangements | IgVH-A, IgVH-B, IgVH-C, IgDH-A, IgDH-B, TCRG A and TCRD rearrangements |
Gross chromosomal alterations | |
 CMA | 47, XY, +8, del(9p24.3-p13.1), del(17p13.3-p11) |
Drug sensitivity | Sensitive to Dex and VCR |
Resistant to DNR, MTX, Ara-c | |
Highly resistant to L-Asp | |
Functional characteristics | |
 Clonality | Yes |
 Tumorigenicity in nude mice | Tumor masses in 3/6 nude mice |