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Fig. 3 | Cancer Cell International

Fig. 3

From: Histone variant MacroH2A1 is downregulated in prostate cancer and influences malignant cell phenotype

Fig. 3

Distribution of MacroH2A1 transcript and protein levels in prostate cancer cell lines. a Transcript levels of total MacroH2A1, MacroH2A1.1 and MacroH2A1.2, assessed by RT-qPCR and normalized to GUSB mRNA levels, in androgen-receptor positive RWPE-1 benign prostate and 22Rv1, LNCaP and VCaP cancer cell lines, and in androgen-receptor negative DU145 and PC-3 prostate cancer cell lines. b Transcript levels of splicing regulators DDX5, DDX17 and QKI, assessed by RT-qPCR and normalized to GUSB, in androgen positive and negative prostate cell lines. c Illustrative images of MacroH2A1 isoforms protein levels in prostate cancer cell lines and in non-malignant prostate cell line RWPE-1. d Fold variation of MacroH2A1.1 and MacroH2A1.2 protein levels, normalized to β-actin, and directly compared to benign prostate cell line RWPE-1. (mean ± SD, n = 3). Dunnet’s pairwise multiple comparisons test: *p < 0.05, **p < 0.01, ***p < 0.001

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