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Fig. 5 | Cancer Cell International

Fig. 5

From: Histone variant MacroH2A1 is downregulated in prostate cancer and influences malignant cell phenotype

Fig. 5

Expression modulation of MacroH2A1.1 isoform in PCa cell lines. a Illustrative images of MacroH2A1 isoforms protein and β-actin protein in MacroH2A1.1 transfected LNCaP cell line (LNCaPMacroH2A1.1OE) and transfected control cell line (LNCaPempty vector) at 72 h post-transfection. b Fold variation of MacroH2A1.1 and MacroH2A1.2 protein levels in LNCaPMacroH2A1.1OE, compared to LNCaPempty vector (mean ± SD, n = 3). Unpaired t test: *p < 0.05, ns = not significant. Impact of MacroH2A1.1 overexpression in cell viability (c) and apoptosis levels (d) after 72 h (mean ± SD, n = 3). Mann–Whitney U-test: *p < 0.05, ***p < 0.001. e Illustrative images of MacroH2A1 isoforms protein after MacroH2A1.1 knockdown, carried out by si-RNA, in DU145 prostate cancer cell line at 72 h’ post-transfection, obtained by Western-Blot. f Fold variation of MacroH2A1.1 and MacroH2A1.2 protein levels in MacroH2A1.1-knockdown DU145 cell line, in comparison to si-scramble DU145 cell line (mean ± SD, n = 3), assessed by western-blot. Unpaired t test: *p < 0.05. Impact of MacroH2A1.1 knockdown in cell viability (g) and apoptosis levels (h) after 72 h (mean ± SD, n = 3). Mann–Whitney U-test: *p < 0.05, **p < 0.01, ***p < 0.001

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