Fig. 1
Apatinib inhibits the growth of PC9GR cells in vitro. a The inhibition rate, as measured by CCK-8 after PC9 and PC9GR cell treated with different concentrations of gefitinib. PC9GR cells have a significantly increased IC50 and demonstrate strong resistance to gefitinib. b Cell viability of PC9, A549, H460, and PC9GR cells after treatment with different concentrations of apatinib, determined by CCK8 assay at the indicated time. PC9GR cells are most sensitive than other cells. c The IC50 values of PC9, A549, H460, and PC9GR cells treated with apatinib where PC9GR cells are significantly lower. d Results of flow cytometry for PC9GR and PC9 cells treated with different concentrations of apatinib for 24 h where increasing fluorescence intensities were seen with increasing concentrations in PC9GR cells but not in the PC9 cells. e Colony formation of PC9GR and PC9 cells at different concentrations of apatinib. Colony numbers are decreasing along with increasing apatinib concentrations in PC9GR cells but there are no changes in PC9 cells. f Bar plots for numbers of colonies from PC9GR and PC9 cells at different concentrations of apatinib. ***Significantly different (p < 0.001) from control cells